Analytical Data
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Gene name
SMARCD3
- Application
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Alternative Names
60 kDa BRG 1/Brm associated factor subunit C; 60 kDa BRG-1/Brm-associated factor subunit C; BAF60C; BRG1 associated factor 60C; BRG1-associated factor 60C; Chromatin remodeling complex BAF60C subunit; CRACD3; Mammalian chromatin remodeling complex BRG1 associated factor 60C; MGC111010; Rsc6p; Smarcd3; SMRD3_HUMAN; SWI/SNF complex 60 kDa subunit C; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily d member 3; SWI/SNF related. matrix associated. actin dependent regulator of chromatin. subfamily d. member 3. isoform 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3; Swp73 like protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6STE5
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Expression Region
2-483 aa
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AA Sequence
DEVAGGA RKATKSKLFE FLVHGVRPGM PSGARMPHQG APMGPPGSPY MGSPAVRPGL APAGMEPARK RPPPGQSQ AQSQGQPVPT APARSRSAKR RKMADKILPQ RIRELVPESQ AYMDLLAFER KLDQTIMRKR VDIQEALKRP MKQKRKLRLY ISNTFNPAKP DAEDSDGSIA SWELRVEGKL LDDPSKQKRK FSSFFKSLVI ELDKDLYGPD NHLVEWHRTP TTQETDGFQV KRPGDLSVRC TLLLMLDYQP PQFKLDPRLA RLLGLHTQSR SAIVQALWQY VKTNRLQDSH DKEYINGDKY FQQIFDCPRL KFSEIPQRLT ALLLPPDPIV INHVISVDPS DQKKTACYDI DVEVEEPLKG QMSSFLLSTA NQQEISALDS KIHETIESIN QLKIQRDFML SFSRDPKGYV QDLLRSQSRD LKVMTDVAGN PEEERRAEFY HQPWSQEAVS RYFYCKIQQR RQELEQSLVV RNT
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Molecular Weight
55.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SMARCD3, a member of the SWI/SNF chromatin-remodeling complex, plays a crucial role in the regulation of gene expression and chromatin dynamics. This protein is known for its involvement in various cellular processes, including differentiation, proliferation, and development. Recent studies have highlighted its significance in cancer biology, where aberrant expression or mutation of SMARCD3 has been linked to tumorigenesis and poor prognosis in several cancer types. The research surrounding the recombinant expression of SMARCD3 is driven by the need to elucidate its structural and functional properties, which are essential for understanding its role in chromatin remodeling and gene regulation. By producing SMARCD3 as a recombinant protein, researchers aim to analyze its biochemical interactions and regulatory mechanisms in greater detail. This includes exploring its binding affinities with other chromatin-associated proteins and assessing its functional implications in cellular contexts. The insights gained from such studies not only contribute to our fundamental understanding of chromatin biology but also open potential avenues for targeted therapies in cancers where SMARCD3 is implicated. Furthermore, the expression of SMARCD3 as a recombinant protein serves as a valuable tool for biochemical assays, structural biology studies, and the development of novel models to study the complexities of chromatin remodeling in health and disease. As such, the investigation of SMARCD3 through recombinant technology is vital for both basic research and clinical applications in oncology, underpinning its importance in the broader field of molecular biology.
