Analytical Data
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基因名
DBF4
- Application
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别名
Activator of S phase kinase like protein 1 ; Activator of S phase kinase-like protein 1; ASK like protein 1; ASK-like protein 1; ASKL1 ; chifb ; Chiffon homolog B; DBF 4B; DBF4 homolog B (S. cerevisiae) ; DBF4 homolog B
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8NFT6
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表达区间
1-674aa
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氨基酸序列
MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMGSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEEDRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF
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分子量
99.66 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DBF4, a crucial regulatory protein, plays an essential role in the activation of cyclin-dependent kinase (CDK) complexes, particularly CDK1 and CDK2, which are pivotal in controlling the cell cycle and DNA replication. As a part of the DBF4-dependent kinase (DDK) complex, DBF4 functions to activate CDC7, facilitating the phosphorylation of proteins essential for the initiation of DNA replication. The study of DBF4 has garnered attention due to its implications in cancer biology; aberrations in its expression and function can lead to uncontrolled cellular proliferation and contribute to tumorigenesis. Furthermore, as DBF4 is involved in the regulation of DNA damage response pathways, understanding its mechanisms provides insights into potential therapeutic targets for cancers with disrupted cell cycle regulation. Recent research has explored the structural properties of DBF4 and its interactions with other cellular partners, revealing the complexity of its regulatory functions. Investigating DBF4's role in various cellular contexts not only enhances our understanding of fundamental cellular processes but also holds promise for the development of innovative cancer treatments. Given its importance, the establishment of recombinant DBF4 proteins enables detailed biochemical and biophysical characterization, expanding our knowledge of how DBF4 influences cell cycle dynamics and contributes to genome stability.
