Analytical Data
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基因名
ZNRF3
- Application
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别名
ZNRF3；KIAA1133；RNF203；E3 ubiquitin-Protein ligase ZNRF3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9ULT6
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表达区间
56-219aa
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氨基酸序列
KETAFVEVVLFESSPSGDYTTYTTGLTGRFSRAGATLSAEGEIVQMHPLGLCNNNDEEDLYEYGWVGVVKLEQPELDPKPCLTVLGKAKRAVQRGATAVIFDVSENPEAIDQLNQGSEDPLKRPVVYVKGADAIKLMNIVNKQKVARARIQHRPPRQPTEYFDM
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分子量
19.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ZNRF3 (Zinc RING Finger 3) is an E3 ubiquitin ligase that plays a crucial role in the regulation of various cellular processes, including cell differentiation, proliferation, and apoptosis. Recently, ZNRF3 has garnered attention for its involvement in the Wnt signaling pathway, a pivotal pathway in developmental biology and tumorigenesis. The aberrant regulation of the Wnt pathway is associated with various cancers, making ZNRF3 a potential therapeutic target. Research has shown that ZNRF3 interacts with Wnt receptors, promoting their degradation and thus acting as a negative regulator of Wnt signaling. Understanding the molecular mechanisms underlying ZNRF3 function can provide insights into its role in cancer biology and other diseases. The production of recombinant ZNRF3 proteins has become essential for characterizing its biochemical properties and interactions, facilitating detailed studies in various experimental contexts. Additionally, the use of recombinant techniques allows researchers to investigate the potential of ZNRF3 as a biomarker or therapeutic target in cancer treatment. Given its significant implications in cell signaling and cancer development, continued research on recombinant ZNRF3 is vital for elucidating its biological functions and developing novel therapeutic strategies.
