Analytical Data
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Gene name
PDK2
- Application
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Alternative Names
PDK2；PDHK2；[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15119
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Expression Region
1-407aa
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AA Sequence
MRWVWALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS
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Molecular Weight
70 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDK2 (Pyruvate Dehydrogenase Kinase 2) is an enzyme that plays a crucial role in regulating cellular energy metabolism by phosphorylating and inactivating the pyruvate dehydrogenase complex. This regulation is vital in influencing various metabolic pathways, including glycolysis and the tricarboxylic acid (TCA) cycle, making PDK2 a significant factor in metabolic disorders, cancer, and insulin resistance. The overexpression of PDK2 has been linked to tumor growth and progression, as it allows cancer cells to preferentially utilize aerobic glycolysis, often referred to as the Warburg effect. Therefore, the study of recombinant PDK2 protein is crucial for understanding its structure-function relationships, along with its regulatory mechanisms within metabolic networks. Research on PDK2 can lead to potential therapeutic interventions targeting metabolic reprogramming in cancer and other metabolic diseases. The development of high-quality recombinant PDK2 allows for detailed biochemical studies, structural analyses, and the screening of small-molecule inhibitors, facilitating the exploration of PDK2 as a promising target for drug discovery. Understanding the molecular basis of PDK2's activity is essential for developing novel strategies to modulate its function and, consequently, its impact on cellular metabolism and disease progression.
