Analytical Data
-
Gene name
APAF1
- Application
-
Alternative Names
APAF1；Methylthioribulose-1-phosphate dehydratase
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O14727-2
-
Expression Region
2-1237aa
-
AA Sequence
MHHHHHHDYKDDDDKDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLT ISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAAL LHDGIPVVSSSSVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEP GWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLM KLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWD SWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSL FVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQN KQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPT KVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTE KNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMH KELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEF LSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEW INKKNITNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETG EKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDE HSETQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMFGHTNSV NHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQED MEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYC DFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSS FLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRL QLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIF QSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQET VKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHD ATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGD DNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAG GYIKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQT LE
-
Molecular Weight
143 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
APAF1 (Apoptosis protein activating factor 1) is a pivotal component of the apoptosome, a multi-protein complex that plays a crucial role in the intrinsic pathway of apoptosis, or programmed cell death. This protein is vital for cellular homeostasis and response to various stressors, including DNA damage and oxidative stress. Mutations or alterations in APAF1 expression have been implicated in various diseases, particularly cancer, where dysregulation of apoptosis contributes to tumorigenesis. Research has increasingly focused on the recombinant expression of APAF1 to enable detailed studies of its structure, function, and interaction with other apoptotic factors. By producing recombinant APAF1, scientists can analyze its conformational changes, investigate its role in caspase activation, and explore potential therapeutic strategies aimed at reactivating apoptotic pathways in cancer cells. The ability to generate purified APAF1 in sufficient quantities also facilitates high-resolution structural studies using techniques such as X-ray crystallography and cryo-electron microscopy, enhancing our understanding of its molecular mechanisms. Overall, studies on recombinant APAF1 offer significant insights into apoptosis regulation and the potential development of novel cancer therapies targeting apoptotic dysregulation.
