Analytical Data
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基因名
USP8
- Application
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别名
USP8；FLRF；NRDP1；E3 ubiquitin-Protein ligase NRDP1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P40818
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表达区间
2-1118aa
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氨基酸序列
PAVASVPKELYLSSSLKDLNKKTEVKPEKISTKSYVHSALKIFKTAEECR LDRDEERAYVLYMKYVTVYNLIKKRPDFKQQQDYFHSILGPGNIKKAVEE AERLSESLKLRYEEAEVRKKLEEKDRQEEAQRLQQKRQETGREDGGTLAK GSLENVLDSKDKTQKSNGEKNEKCETKEKGAITAKELYTMMTDKNISLII MDARRMQDYQDSCILHSLSVPEEAISPGVTASWIEAHLPDDSKDTWKKRG NVEYVVLLDWFSSAKDLQIGTTLRSLKDALFKWESKTVLRNEPLVLEGGY ENWLLCYPQYTTNAKVTPPPRRQNEEVSISLDFTYPSLEESIPSKPAAQT PPASIEVDENIELISGQNERMGPLNISTPVEPVAASKSDVSPIIQPVPSI KNVPQIDRTKKPAVKLPEEHRIKSESTNHEQQSPQSGKVIPDRSTKPVVF SPTLMLTDEEKARIHAETALLMEKNKQEKELRERQQEEQKEKLRKEEQEQ KAKKKQEAEENEITEKQQKAKEEMEKKESEQAKKEDKETSAKRGKEITGV KRQSKSEHETSDAKKSVEDRGKRCPTPEIQKKSTGDVPHTSVTGDSGSGK PFKIKGQPESGILRTGTFREDTDDTERNKAQREPLTRARSEEMGRIVPGL PSGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSAPPSTPPTHKAKPQ IPAERDREPSKLKRSYSSPDITQAIQEEEKRKPTVTPTVNRENKPTCYPK AEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHL ADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFK ITIGKINDQFAGYSQQDSQELLLFLMDGLHEDLNKADNRKRYKEENNDHL DDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTFEAFMY LSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIW KLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKNNLKKY NLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVKSSAA YILFYTSLGPRVTDVAT
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分子量
128 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
USP8 (ubiquitin-specific protease 8) is a member of the USP family of deubiquitinating enzymes that play crucial roles in regulating protein stability and cellular signaling by removing ubiquitin moieties from target proteins. Dysregulation of USP8 has been implicated in various diseases, including cancers and endocrine disorders, particularly in Cushing's disease, where mutations in USP8 lead to aberrant activation of the EGFR pathway. The study of recombinant USP8 protein has gained significant traction due to its potential as a therapeutic target and its importance in understanding the mechanisms of ubiquitin-mediated proteolysis. Researchers aim to elucidate the structural and functional characteristics of USP8 to develop specific inhibitors that can modulate its activity, thereby offering new avenues for treatment in diseases associated with USP8 dysfunction. Furthermore, the production and characterization of recombinant USP8 provide essential insights into its enzymatic mechanisms, substrate specificity, and interaction with other cellular components. Overall, the comprehensive investigation of USP8 through recombinant protein studies not only deepens our understanding of ubiquitination pathways but also paves the way for innovative therapeutic strategies targeting this crucial enzyme in various pathological contexts.
