Analytical Data
-
Gene name
FBXO28
- Application
-
Alternative Names
CENP 30; Centromere protein 30; F box only protein 28; F box protein 28; F-box only protein 28; Fbx28 ; FBX28_HUMAN; FBXO28; FLJ10766; KIAA0483; OTTHUMP00000035597
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9NVF7
-
Expression Region
1-368aa
-
AA Sequence
MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPGSQALPAPALAPDQLPQNNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEYFDEKIVPILKRKLPGSDVSGRLMGSPPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVTVLRREISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGNSSGSGQNEESPRKRKKATEAIDSLRKSKRLRNRK
-
Molecular Weight
67.5 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FBXO28, a member of the F-box protein family, plays a critical role in the ubiquitin-proteasome system, which governs protein degradation and regulates various cellular processes. Its unique structure, featuring an F-box motif that facilitates interaction with Skp1, allows FBXO28 to target specific substrates for ubiquitination, influencing key biological functions such as cell cycle regulation, differentiation, and response to stress. Recent studies have suggested that FBXO28 may have implications in cancer biology, as altered expression levels have been observed in various tumor types. The understanding of FBXO28's substrate specificity and its mechanism of action remains limited, prompting researchers to explore its potential as a therapeutic target. Additionally, the development of recombinant FBXO28 proteins may aid in elucidating its functional roles and interactions in cellular pathways. Investigating FBXO28 through recombinant protein techniques underscores its significance in post-translational modifications, providing insights into how dysregulation of this pathway could contribute to disease progression. Overall, the research surrounding FBXO28 is vital for uncovering new avenues in cancer treatment and understanding the broader implications of ubiquitin-mediated signaling in health and disease.
