Analytical Data
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基因名
GLIS3
- Application
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别名
GLIS3; ZNF515; Zinc finger protein GLIS3; GLI-similar 3; Zinc finger protein 515
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8NEA6
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表达区间
1-774aa
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氨基酸序列
MVQRLGLISPPASQVSTACNQISPSLQRAMNAANLNIPPSDTRSLISRESLASTTLSLTESQSASSMKQEWSQGYRALPSLSNHGSQNGLDLGDLLSLPPGTSMSSNSVSNSLPSYLFGTESSHSPYPSPRHSSTRSHSARSKKRALSLSPLSDGIGIDFNTIIRTSPTSLVAYINGSRASPANLSPQPEVYGHFLGVRGSCIPQPRPVPGSQKGVLVAPGGLALPAYGEDGALEHERMQQLEHGGLQPGLVNHMVVQHGLPGPDSQPAGLFKTERLEEFPGSTVDLPPAPPLPPLPPPQGPPPPYHAHAHLHHPELGPHAQQLALPQATLDDDGEMDGIGGKHCYRWIDCSALYDQQEELVRHIEKVHIDQRKGEDFTCFWAGCPRRYKPFNARYKLLIHMRVHSGEKPNKCTFEGCEKAFSRLENLKIHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDTKPYACQIPGCTKRYTDPSSLRKHVKAHSSKEQQARKKLRSSTEVHPDLLTDCLTVQSLQPATSPRDAAAEGTVGRSPGPGPDLYSAPIFSSNYSSRSGTAAGAVPPPHPVSHPSPGHNVQGSPHNPSSQLPPLTAVDAGAERFAPSAPSPHHISPRRVPAPSSILQRTQPPYTQQPSGSHLKSYQPETNSSFQPNGIHVHGFYGQLQKFCPPHYPDSQRIVPPVSSCSVVPSFEDCLVPTSMGQASFDVFHRAFSTHSGITVYDLPSSSSSLFGESLRSGAEDATFLQISTVDRCPSQLSSVYTEG
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分子量
85.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GLIS3, a member of the GLI-similar protein family, is a transcription factor that plays a pivotal role in various biological processes, including cellular differentiation, development, and metabolism. Initially identified for its involvement in the regulation of insulin signaling and the development of pancreatic beta cells, GLIS3 has garnered significant attention in the context of diabetes and other metabolic disorders. Mutations in the GLIS3 gene have been linked to a rare genetic condition known as Neonatal Diabetes Mellitus, highlighting its essential role in glucose homeostasis. Beyond its implications in diabetes, recent studies have begun to uncover its broader functions in stem cell biology and organ development. Given its multifaceted roles, the development and characterization of GLIS3 recombinant proteins facilitate the exploration of its intricate signaling pathways and interactions with other cellular molecules. Such studies could provide insights into the potential therapeutic applications of GLIS3, particularly in regenerative medicine and the treatment of endocrine disorders. Understanding its structure and function through recombinant protein studies could pave the way for novel interventions aimed at modulating GLIS3 activity, ultimately contributing to the development of targeted therapies for metabolic diseases.
