Analytical Data
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Gene name
MPO
- Application
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Alternative Names
MPO；Myeloperoxidase
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Species
Human
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Source
E. coli
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Tag
N-terminal His-tag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P05164
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Expression Region
49~745aa
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AA Sequence
AAPAVLGEVDTSLVLSSMEEAKQLVDKAYKERRESIKQRLRSGSASPIELLSYFKQPVAATRTAVRAADYLHVALDLLERKLRSLWRRPFNVTDVLTPAQLNVL SKSSGCAYQDVGVTCPEQDKYRTITGMCNNRRSPTLGASNRAF VRWLPAEYEDGF SLPYGWTPGVKRNGFPVALARAVSNEIVRFPTDQLTPDQERSLMF MQWGQLLDHDLDFTPEPAARASFVTGVNCETSCVQQPPCFPLKIPPNDPRIKNQADCIPFFRSCPACPGSNITIRNQINALTSFVDASMVYGSEEPLARNLRNMISNQLGLLAVNQRFQDNGRALLPFDNLHDDPCLLTNRSARIPCFLAGDTRSSEMPELTSMHTLLLREHNRLATELKSLNPRWDGERLYQEARKIVGAMVQIITYRDYLPLVLGPTAMRKYLPTYRSYNDSVDPRIANVFTNAFRYGHTLIQPFIFRLDNRYQPMEPNPRVPLSRVFFASWRVVLEGGIDPILRGLMATPAKLNRONOIAVDEIRERLFEQVIRIGLDLPALNMIQRSRDHGLPGYNAWRRFCGLPQPETVGQLGTVLRNLKLARKLMEQYGTPNNIDIWMGGVSEPLKRKGRVGPLLACIIGTQFRKLRDGDRFWWENEGVF SMQQRQALAQI SLPRIICDNTGITTVSKNNIFISNSYPRDF VNCSTLPALNLASWREAS
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Molecular Weight
82.7kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
MPO (myeloperoxidase) is a heme enzyme primarily found in neutrophils, playing a crucial role in the immune response by producing hypochlorous acid from hydrogen peroxide and chloride ions. This antimicrobial agent aids in the destruction of pathogens during inflammation. Research into MPO has expanded beyond its traditional roles, uncovering its involvement in various diseases, including cardiovascular diseases, cancer, and autoimmune disorders. The enzyme's pro-inflammatory effects have triggered interest in its potential as a biomarker for these conditions. However, the study of MPO also presents challenges, such as its complex structure and the difficulty of obtaining large quantities of functional protein for experimental purposes. Therefore, the development of recombinant MPO through techniques like genetic engineering is gaining momentum. Recombinant MPO can be produced in various systems, enabling researchers to investigate its functionality, interactions, and potential therapeutic applications more thoroughly. This approach not only allows for the detailed study of MPO's enzymatic properties but also offers insights into how it can be modulated for therapeutic benefit, highlighting its significance in both basic research and clinical applications.
