Analytical Data
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基因名
CD40
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简介
CD40 protein is a TNFSF5/CD40LG receptor that transduces signals through TRAF6 and MAP3K8-mediated pathways, activates ERK in macrophages and B cells, and induces immunoglobulin secretion. CD40 exists as monomers and homodimers and interacts with TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF5, and TRAF6). CD40 Protein, Human (HEK293, C-His) is the recombinant human-derived CD40 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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生物活性
1. Measured by its binding ability in a functional ELISA. Immobilized human CD40 at 2 μg/mL (100 μL/well) can bind human CD40L with a linear range of 15.6-500 ng/mL. 2. Loaded Abiprubart (HY-P990726) on AHC2 biosensor, can bind CD40 Protein, Human (HEK293, His) with an affinity constant of 2.792E-09 M as determined in BLI assay. Loaded Abiprubart (HY-P990726) on AHC2 biosensor, can bind CD40 Protein, Human (HEK293, His) with an affinity constant of 2.792E-09 M as determined in BLI assay.
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别名
Tumor Necrosis Factor Receptor Superfamily member 5; Bp50; CD40L Receptor; CDw40; TNFRSF5
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P25942-1
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表达区间
E21-R193
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蛋白长度
Extracellular Domain
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分子量
28-32 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD40 is a co-stimulatory protein found on the surface of various immune cells, including B cells, dendritic cells, and macrophages. It plays a crucial role in regulating immune responses and facilitating T cell activation, making it a significant target for therapeutic intervention in various diseases. The study of recombinant CD40 proteins has gained momentum in immunology due to their potential in vaccine development, cancer therapy, and autoimmune disease treatment. Recombinant CD40 and its ligands can enhance the activation and proliferation of immune cells, improving their ability to respond to pathogens or tumors. By examining the structure and function of CD40, researchers aim to understand its mechanisms and optimize its use in clinical applications. Additionally, exploring CD40's interactions with other immune pathways can help identify new strategies for modulating immune responses. Given its pivotal role in promoting immunity and maintaining homeostasis, CD40 remains a focal point of research aimed at harnessing its properties for therapeutic benefits. The advancement of recombinant DNA technology has facilitated the production of this protein, allowing for detailed study and application in various biomedical fields, thus highlighting the importance of ongoing research into CD40 recombinant proteins.
