Analytical Data
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基因名
DLL1
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简介
DLL1 protein is a transmembrane ligand for NOTCH1, NOTCH2 and NOTCH3 receptors and participates in cis- and trans-interactions. Trans interactions trigger mechanical forces through clathrin-mediated endocytosis, involving ligand ubiquitination, EPN1 interactions, and actin polymerization. Delta-like protein 1/DLL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Delta-like protein 1/DLL1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is approximately 0.4845-0.6340 μg/mL. Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is approximately 0.6340 μg/mL, corresponding to a specific activity is 1577.2871 units/mg.
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别名
Delta-like protein 1; Delta1; Dll1-EC; Dll1-TMIC; Dll1
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种属
Mouse
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表达系统
HEK293
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标签
C-10*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q61483
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表达区间
Q18-Q516
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氨基酸序列
QVWSSGVFELKLQEFVNKKGLLGNRNCCRGGSGPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGAGIDPAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLTTQRHLTVGEEWSQDLHSSGRTDLRYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGDRGEKMCDPGWKGQYCTDPICLPGCDDQHGYCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCRNGATCTNTGQGSYTCSCRPGYTGANCELEVDECAPSPCKNGASCTDLEDSFSCTCPPGFYGKVCELSAMTCADGPCFNGGRCSDNPDGGYTCHCPLGFSGFNCEKKMDLCGSSPCSNGAKCVDLGNSYLCRCQAGFSGRYCEDNVDDCASSPCANGGTCRDSVNDFSCTCPPGYTGKNCSAPVSRCEHAPCHNGATCHQRGQRYMCECAQGYGGPNCQ
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蛋白长度
Partial
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分子量
58-60 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DLL1 (Delta-like ligand 1) is an important component of the Notch signaling pathway, which plays a critical role in various biological processes, including cell differentiation, proliferation, and apoptosis. DLL1 acts as a ligand that binds to Notch receptors on adjacent cells, thereby influencing cell fate decisions during embryonic development and tissue homeostasis. Research into DLL1 recombinant proteins has gained momentum due to their potential therapeutic applications in regenerative medicine, cancer immunotherapy, and tissue engineering. By producing DLL1 in a recombinant form, researchers can better study its structural properties and functional mechanisms, paving the way for targeted therapies that manipulate the Notch pathway. Additionally, understanding the precise interactions of DLL1 with its receptors and other signaling molecules can provide insights into various diseases characterized by aberrant cell signaling, such as cancers and developmental disorders. This growing interest in DLL1 recombinant proteins underscores their significance as both a biochemical tool and a potential therapeutic agent, driving further exploration of their role in cell communication and developmental biology.
