Recombinant Human CD20/MS4A1 Protein, His & Trx

CD20/MS4A1

CD20/MS4A1 protein is a B lymphocyte membrane protein that plays a crucial regulatory role in cellular calcium influx, which is essential for the development, differentiation and activation of B lymphocytes. As part of a store-operated calcium (SOC) channel, it promotes calcium influx upon B cell receptor/BCR activation. CD20/MS4A1 Protein, Human (Trx-His) is the recombinant human-derived CD20/MS4A1 protein, expressed by E. coli , with N-Trx, N-6*His labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

1. Measured by its binding ability in a functional ELISA. Immobilized Human CD20, at 2 μg/mL (100 μL/well) can bind Anti-CD20 antibody. The ED50 for this effect is 0.3077-0.5525 μg/mL. 2. Loaded Tositumomab (HY-P99326) on AHC2 biosensor, can bind CD20/MS4A1 Protein, Human (Trx-His) with an affinity constant of <1.000E-11 M as determined in BLI assay. Loaded Tositumomab (HY-P99326) on AHC2 biosensor, can bind CD20/MS4A1 Protein, Human (Trx-His) with an affinity constant of <1.000E-11 M as determined in BLI assay.

rHuB-lymphocyte antigen CD20/CD20, Trx-His; MS4A1; CD20; MS4A-1; B1; Bp35; CVID5; LEU-16; MS4A2; S7

Human

E. coli

N-Trx;N-6*His

Greater than 90% as determined by SDS-PAGE.

P11836-1

I141-S188

Extracellular Domain

20.91 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.