Analytical Data
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Gene name
SHH
- Application
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Biological Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1110-0.1969 μg/mL. Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1110 μg/mL, corresponding to a specific activity is 9009.009 units/mg.
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Alternative Names
rMuShh; HHG-1; ShhNC
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Species
Mouse
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Source
HEK293
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q62226
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Expression Region
C25-G198
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Protein Length
Partial
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Molecular Weight
20.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Sonic Hedgehog (SHH) is a crucial signaling molecule in developmental biology, primarily known for its role in the regulation of cell growth, differentiation, and patterning during embryonic development. The SHH pathway is integral for the formation of various tissues and organs, particularly the nervous system, where it influences neural stem cell behavior and neurogenesis. Abnormal SHH signaling has been implicated in various developmental disorders and diseases, including congenital malformations and certain types of cancer, such as medulloblastoma and basal cell carcinoma. Due to its significant role in both normal physiology and pathological conditions, research into SHH has focused on understanding its structure, function, and the mechanisms governing its signaling pathways. The ability to produce recombinant SHH protein has advanced these studies, allowing for detailed investigations into its biochemical properties, interaction with receptors, and downstream signaling effects. Using recombinant SHH in experimental models has enabled researchers to dissect its roles in various biological processes and to explore potential therapeutic interventions targeting SHH-related pathways. Overall, the study of SHH and its recombinant forms is vital for elucidating fundamental aspects of developmental biology and for developing strategies to address related diseases.
