Analytical Data
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基因名
PPM1A
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简介
PPM1A is a multifunctional enzyme that negatively regulates multiple signaling pathways. It significantly attenuates TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export, and signal termination. PPM1A Protein, Human (His) is the recombinant human-derived PPM1A protein, expressed by E. coli , with C-6*His labeled tag.
- Application
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别名
Protein Phosphatase 1A; Protein Phosphatase 2C Isoform Alpha; PP2C-Alpha; Protein Phosphatase IA; PPM1A; PPPM1A
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种属
Human
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表达系统
E. coli
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P35813-1
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表达区间
G2-W382
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氨基酸序列
GAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW
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蛋白长度
Full Length of Isoform-1 Mature Protein
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分子量
45.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent 1A) is a serine/threonine phosphatase that plays a critical role in various cellular processes, including cell cycle regulation, apoptosis, and stress responses. Research on PPM1A has gained momentum due to its involvement in several pathological conditions, such as cancer and cardiovascular diseases. PPM1A is known to dephosphorylate several key proteins, thereby modulating signaling pathways that are crucial for cell growth and survival. For instance, its activity can influence the MAPK/ERK signaling pathway, which is frequently dysregulated in tumors. Furthermore, PPM1A has been identified as a key regulator of the tumor suppressor p53 and the oncogene c-Myc, highlighting its potential as a therapeutic target in cancer treatment. The exploration of PPM1A recombinant protein enables researchers to delve deeper into its biochemical properties, regulatory mechanisms, and interaction with various substrates. By producing PPM1A in a recombinant expression system, scientists can obtain large quantities of the protein for functional studies, providing insights into its role in disease and aiding the development of novel therapeutic strategies. Overall, the ongoing research on PPM1A not only enhances our understanding of fundamental cellular processes but also opens new avenues for therapeutic intervention in diseases characterized by aberrant signaling pathways.
