Analytical Data
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Gene name
OX40 Ligand/TNFSF4
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简介
OX40 Ligand/TNFSF4 Protein is a cytokine that binds specifically to TNFRSF4 and acts as a potent T-cell co-stimulator when OX40 Ligand is expressed in trimer form, promoting proliferation and cytokine production. Interactions with TNFRSF4 contribute to complex regulation, which is essential for regulating T cell function and enabling effective and controlled immune activation. OX40 Ligand/TNFSF4 Protein, Human (HEK293) is the recombinant human-derived OX40 Ligand/TNFSF4 protein, expressed by HEK293, which is unlabeled and a monomeric protein.
- Application
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Biological Activity
Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. The ED50 for this effect is 3.527 ng/mL, corresponding to a specific activity is 2.836×105 U/mg. Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. The ED50 for this effect is 3.527 ng/mL, corresponding to a specific activity is 2.836×105 U/mg.
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Alternative Names
OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L; TXGP1; Glycoprotein Gp34
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Species
Human
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Source
HEK293
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P23510-1
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Expression Region
Q51-L183
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Protein Length
Extracellular Domain
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Molecular Weight
18-30 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
OX40 Ligand (OX40L), also known as TNFSF4, is a member of the tumor necrosis factor superfamily and plays a crucial role in regulating immune responses, particularly in T cell activation and survival. OX40L is primarily expressed on antigen-presenting cells and interacts with its receptor OX40 on T cells, leading to enhanced T cell proliferation, survival, and memory formation. The OX40/OX40L pathway has garnered significant interest in cancer immunotherapy, as it can bolster anti-tumor T cell responses. Furthermore, dysregulation of this pathway has been linked to various autoimmune diseases and inflammatory conditions. Research on recombinant OX40L protein involves its production, characterization, and assessment of its effects on T cell function in both in vitro and in vivo models. Understanding the structure-function relationship of OX40L and its interaction with OX40 provides insight into potential therapeutic applications, including the development of OX40 agonists for enhancing T cell responses against tumors or the design of antagonists to mitigate excessive immune activation in autoimmune diseases. Overall, the study of recombinant OX40 Ligand/TNFSF4 is vital for unveiling new strategies in immunotherapy and advancing the field of immune modulation.
