Analytical Data
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Gene name
CHI3L1
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简介
The CHI3L1 protein is a binding lectin that lacks chitinase activity. CHI3L1 contributes to tissue remodeling, cellular adaptation to environmental changes, and T helper type 2 inflammatory responses. CHI3L1 also controls hyperoxia-induced injury, inflammation, and epithelial cell apoptosis. CHI3L1 Protein, Human (HEK293, His) is the recombinant human-derived CHI3L1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 0.6-3.0 μg/mL. Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 2.603 μg/mL, corresponding to a specific activity is 384.1721 units/mg.
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Alternative Names
rHuChitinase-3-like protein 1/CHI3L1, His; Chitinase-3-Like protein 1; 39 kDa Synovial Protein; Cartilage Glycoprotein 39; CGP-39; GP-39; hCGP-39; YKL-40; CHI3L1
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P36222
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Expression Region
Y22-T383
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AA Sequence
YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT
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Protein Length
Full Length of Mature Protein
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Molecular Weight
41-46 kDa band in SDS-PAGE under reducing conditions.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHI3L1, also known as chitinase 3-like 1 or YKL-40, is a glycoprotein that has garnered significant attention in recent years due to its involvement in various pathological processes, particularly in inflammation and tissue remodeling. This protein is primarily expressed in macrophages, chondrocytes, and other cell types in response to pro-inflammatory stimuli. Elevated levels of CHI3L1 have been implicated in different diseases, including cancer, asthma, and autoimmune disorders, where it often serves as a biomarker for disease progression and severity. Research has suggested that CHI3L1 plays a crucial role in modulating inflammation and has potential implications for therapeutic intervention. The protein's ability to bind to chitin and its structural similarities to chitinases suggest that it may influence extracellular matrix dynamics and tissue repair processes. Consequently, recombinant CHI3L1 has emerged as a valuable tool for studying its biological functions, mechanisms of action, and potential clinical applications. Understanding the role of CHI3L1 in various diseases could lead to novel therapeutic strategies and improve diagnostic capabilities in inflammatory and degenerative conditions. The ongoing exploration of its multifaceted roles highlights the significance of CHI3L1 as a target for both research and clinical advancements.
