Analytical Data
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基因名
Latent TGF beta 1/TGFB1
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简介
Transforming growth factor beta-1 proprotein is the precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains. TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) is a recombinant protein (L30-S390) produced by HEK293 cells with His tag[1][2].
- Application
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生物活性
1. Immobilized Mouse Latent TGF beta 1, His Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, hFc Tag with the EC50 of 0.39-0.43 μg/mL determined by ELISA. 2. Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is ≤0.2066 ng/mL, corresponding to a specific activity is ≥4.84×106 units/mg. Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.1173 ng/mL, corresponding to a specific activity is 8.525×106 units/mg.
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别名
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1
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种属
Mouse
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表达系统
HEK293
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标签
N-His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
P04202
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表达区间
L30-S390
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氨基酸序列
LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYEKTKDISHSIYMFFNTSDIREAVPEPPLLSRAELRLQRLKSSVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNKLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
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蛋白长度
Full Length of Mature Protein
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分子量
40-50 kDa & 14-15 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Latent TGF-beta 1 (TGFB1) is a member of the TGF-beta superfamily, recognized for its crucial role in regulating various cellular processes, including cell proliferation, differentiation, and immune responses. It exists in a latent form, sequestered in the extracellular matrix, which prevents premature activation. The activation of latent TGF-beta 1 is a fundamental step in various physiological and pathological processes, such as wound healing, fibrosis, and cancer progression. Given its significant implications in these areas, recombinant forms of latent TGF-beta 1 have been generated for research purposes. These recombinant proteins serve as valuable tools for elucidating the mechanisms of TGF-beta activation and signaling pathways, which can lead to the development of novel therapeutic strategies targeting TGF-beta related diseases. Additionally, understanding the structural and functional aspects of latent TGF-beta 1 is essential for designing effective interventions to modulate its activity in various disease states. Overall, the study of recombinant latent TGF-beta 1 contributes to advancing our knowledge in cellular biology and therapeutic applications.
