Analytical Data
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基因名
Apolipoprotein E/APOE
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简介
Apolipoprotein E/APOE is essential for lipid transport between organs and is a key component of lipoproteins such as chylomicrons and high-density lipoprotein. It binds to cell receptors such as LDLR and VLDLR to promote lipoprotein uptake, and has heparin-binding activity to interact with cell surface proteoglycans. Apolipoprotein E/APOE Protein, Rabbit (His-SUMO) is the recombinant Rabbit-derived Apolipoprotein E/APOE protein, expressed by E. coli , with N-10*His, N-SUMO labeled tag.
- Application
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别名
APOEApolipoprotein E; Apo-E
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种属
Rabbit
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表达系统
E. coli
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标签
N-10*His;N-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P18287
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表达区间
T20-Q311
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氨基酸序列
TEQEVEVPEQARWKAGQPWELALGRFWDYLRWVQSLSDQVQEELLSSQVTQELTMLMEETMKEVKAYKSELEEQLSPMAQEHRARLSKELQVAGALEADMEDVCNRLAQYRGEAQAMLGQSTEELARAFSSHLRKLRKRLLRDAEDLQKRMAVYGAGAREGAERGVSAVRERLGSRLERGRLRVATVGTLAGRPLRERAQAWGERLRGHLEEVGSRARDRLNEVREQVEEVRVKVEEQAPQMRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKLQAAMPSKAPAAAPIENQ
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蛋白长度
Full Length of Mature Protein
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Apolipoprotein E (APOE) is a pivotal protein involved in lipid metabolism and neurobiology, primarily recognized for its role in the transport and clearance of lipoproteins in the bloodstream. The APOE gene exhibits three major alleles: ε2, ε3, and ε4, with the ε4 variant being a significant genetic risk factor for Alzheimer's disease and other neurodegenerative disorders. Research into recombinant APOE protein has gained momentum due to its potential in elucidating the mechanisms underlying these diseases and its therapeutic implications. Recombinant APOE proteins are crucial for studying their structure-function relationships, lipid-binding properties, and interactions with receptors like the LDL receptor and ApoE receptors. By producing and characterizing these proteins in vitro, researchers aim to understand how variations in APOE affect lipid metabolism, amyloid plaque formation, and neural repair processes. Furthermore, the recombinant forms can be employed in animal models to assess their influence on disease progression and treatment outcomes. This growing body of research not only enhances our understanding of APOE's biological roles but also opens avenues for developing targeted therapies aimed at mitigating the effects of APOE-related pathologies. As Alzheimer’s disease continues to be a pressing global health issue, the development and application of recombinant APOE proteins represent a promising direction in the quest for effective diagnostics and treatments.
