Analytical Data
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基因名
Osteopontin/OPN
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简介
Osteopontin/OPN Protein, a crucial non-collagenous bone protein, binds strongly to hydroxyapatite, integral to the mineralized matrix.It likely plays a vital role in mediating cell-extracellular matrix interactions.Moreover, as a cytokine, OPN efficiently induces interferon-gamma and interleukin-12 production while inhibiting interleukin-10, pivotal in promoting type I immunity.Osteopontin/OPN Protein, Mouse (HEK293, His) is the recombinant mouse-derived Osteopontin/OPN protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 105 cells/well are added to Recombinant Human Osteopontin coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is <0.3 μg/mL. Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is 0.1425 μg/mL, corresponding to a specific activity is 7017.5439 units/mg
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别名
Osteopontin; Bone sialoprotein 1; Nephropontin; SPP-1; BNSP; OPN; SPP1; Uropontin; BSP-1; ETA-1; BSPI
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种属
Mouse
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P10923
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表达区间
L17-N294
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蛋白长度
Full Length of Mature Protein
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分子量
50-70 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Osteopontin (OPN) is a glycoprotein that plays a significant role in bone remodeling, immune responses, and various physiological and pathological processes. Its involvement in cellular signaling pathways and its ability to interact with different cell types make it a key player in inflammation, tissue repair, and tumor progression. Research on recombinant OPN protein has gained traction due to its potential applications in biomedical fields, including regenerative medicine and cancer therapy. Recombinant technology facilitates the production of OPN in sufficient quantities for in vitro and in vivo studies, enabling researchers to investigate its functional roles under various conditions. Moreover, the study of OPN is crucial for understanding diseases like osteoporosis, cardiovascular disorders, and certain cancers, where its expression levels often correlate with disease progression and outcomes. By elucidating the molecular mechanisms through which osteopontin operates, scientists aim to develop targeted therapies that can modulate its activity, enhancing therapeutic strategies and improving patient prognosis. The growing body of evidence highlighting OPN's multifaceted roles in both health and disease underscores the importance of further exploring its pathways and therapeutic potential.
