Analytical Data
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Gene name
EXOSC9
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简介
EXOSC9 is a noncatalytic RNA exosome complex component that critically drives 3'->5' exoribonuclease activity and contributes to a variety of cellular RNA processing and degradation events. In the nucleus, it helps stabilize the maturation of RNA species and eliminates processing by-products, non-coding transcripts and defective mRNAs, thereby inhibiting their cytoplasmic export. EXOSC9 Protein, Human (sf9, His) is the recombinant human-derived EXOSC9 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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Alternative Names
Exosome complex component RRP45; Autoantigen PM/Scl 1; PM/Scl-75; PMSCL1
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Species
Human
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Source
Baculovirus
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q06265-1
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Expression Region
M1-N439
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Protein Length
Full Length of Isoform-1
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EXOSC9, a core component of the exosome complex, plays a vital role in RNA processing and degradation, influencing cellular RNA metabolism and gene regulation. As an essential protein involved in maintaining RNA homeostasis, its dysfunction has been implicated in a variety of diseases, particularly those affecting the nervous system, such as neurodegenerative disorders. The study of EXOSC9 recombinant protein is critical for understanding its structural and functional characteristics, as well as its interactions with other proteins and RNA substrates. Furthermore, elucidating the biochemical pathways in which EXOSC9 participates could provide insights into the molecular mechanisms underlying its role in disease pathogenesis. Recent advances in recombinant protein technology enable the expression and purification of EXOSC9, facilitating the investigation of its enzymatic activities and potential therapeutic applications. Researchers are particularly interested in delineating how mutations in the EXOSC9 gene contribute to disease phenotypes and exploring the potential of targeting the exosome complex in drug development. By leveraging tools such as crystallography and cryo-electron microscopy, studies aim to reveal the three-dimensional structure of EXOSC9 and its complexes, offering valuable information for the design of small molecules that could modulate its activity. Overall, the investigation of EXOSC9 recombinant protein is a promising area of research that bridges fundamental molecular biology and clinical relevance, thereby enhancing our understanding of cellular RNA regulation and its implications in health and disease.
