Analytical Data
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Gene name
Mucolipin-1/MCOLN1
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简介
The TRPML1 protein is a nonselective cation channel that regulates membrane trafficking and metal homeostasis. It promotes the release of Ca(2+) in late endosomes and lysosomes, affecting organelle fusion, exocytosis and autophagy. Mucolipin-1/MCOLN1 Protein, Human (HEK293, StrepⅡ, FLAG) is the recombinant human-derived TRPML1 protein, expressed by HEK293 , with N-Strep, C-Flag labeled tag.
- Application
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Alternative Names
MCOLN1 (ML4, TRPML1); Mucolipin-1; MG-2; Mucolipidin; Transient receptor potential channel mucolipin 1 (TRPML1)
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Species
Human
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Source
HEK293
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Tag
N-StrepⅡ;C-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9GZU1
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Expression Region
M1-N580
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Mucolipin-1 (MCOLN1) is a protein encoded by the MCOLN1 gene, which plays a crucial role in the lysosomal function and cellular ion homeostasis. It is a member of the mucolipin family of proteins and is primarily involved in the transport of cations, such as calcium, in and out of lysosomes. Dysfunction of MCOLN1 has been implicated in mucolipidosis type IV, a rare autosomal recessive lysosomal storage disease that leads to severe neurological and ocular abnormalities. Researchers have focused on understanding the structure and function of MCOLN1 to uncover its role in lysosomal dynamics and disease pathology. The recombinant expression of MCOLN1 has been pivotal for these studies, enabling scientists to investigate its ion channel properties, mechanisms of dysfunction, and potential therapeutic targets. By producing and characterizing the recombinant protein, researchers aim to elucidate the molecular basis of mucolipidosis and develop strategies for intervention. This research not only enhances our understanding of lysosomal biology but also provides insights into potential therapeutic approaches for related disorders and advancing gene therapy techniques. Overall, the investigation of MCOLN1 as a recombinant protein serves as a fundamental step towards developing effective treatments for lysosomal storage disorders.
