Analytical Data
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Gene name
ANKFY1
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简介
ANKFY1 is a proposed Rab5 effector that critically participates in endosomal dynamics by binding to PI(3)P, promoting homotypic early endosomal fusion and participating in heterotypic fusion. It plays a crucial role in macropinocytosis, is dependent on Rab5-GTP, and is essential for the correct localization of activated tyrosine kinase receptors such as PDGFRB. ANKFY1 Protein, Human (sf9, His, Strep) is the recombinant human-derived ANKFY1 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
- Application
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Alternative Names
ANKFY1; Rabankyrin-5; Rank-5; Ankyrin repeat and FYVE domain-containing protein 1; Ankyrin repeats hooked to a zinc finger motif
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Species
Human
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Source
Baculovirus
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Tag
N-StrepⅡ;N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9P2R3
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Expression Region
A2-S1169
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Protein Length
Full Length of Mature Protein
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ANKFY1 is a protein that has garnered increasing attention in recent years due to its potential role in various biological processes and its involvement in human diseases. The gene encoding ANKFY1 is known for its ankyrin-repeat and FYVE domains, which suggest functions related to protein-protein interactions and membrane trafficking. Research has indicated that ANKFY1 may play a crucial role in cellular processes such as autophagy, endocytosis, and immune responses. Mutations and dysregulation of ANKFY1 expression have been linked to certain pathological conditions, including neurodegenerative diseases and metabolic disorders. Consequently, understanding the structure and function of ANKFY1 is essential for elucidating its biological relevance and therapeutic potential. Efforts in recombinant protein expression and purification have enabled detailed studies of ANKFY1, fostering insights into its mechanistic roles and interactions with other cellular components. This research lays the groundwork for exploring ANKFY1 as a potential biomarker or therapeutic target, emphasizing the importance of continued investigation into its functional implications in health and disease.
