Analytical Data
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Gene name
PTH1R
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human PTH1R is used at 2 μg/mL, the concentration of biotinylated human PTHrP that produces 50% of the optimal binding response is found to 84.57 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human PTH1R is used at 2 μg/mL, the concentration of biotinylated human PTHrP that produces 50% of the optimal binding response is found to 84.57 ng/mL.
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Alternative Names
Parathyroid hormone/parathyroid hormone-related peptide receptor; PTH/PTHrP type I receptor; PTH/PTHr receptor; Parathyroid hormone 1 receptor; PTH1 receptor; PTH1R
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q03431
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Expression Region
Y23-M189
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Protein Length
Partial
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Molecular Weight
25-35 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Parathyroid hormone 1 receptor (PTH1R) is a crucial receptor involved in bone metabolism and calcium homeostasis, primarily mediating the actions of parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHrP). Abnormalities in PTH1R signaling are associated with a variety of bone disorders, including osteoporosis and the rare genetic condition known as Blomstrand's osteochondrodysplasia, characterized by severe skeletal abnormalities. Understanding the structure and function of the PTH1R is essential for elucidating these conditions and developing targeted therapies. Recent advances in recombinant protein technology have enabled the production of functional PTH1R proteins, which are vital for investigating receptor pharmacodynamics and designing novel ligands. Studies using these recombinant proteins facilitate the mapping of PTH1R interaction sites and the characterization of downstream signaling pathways, ultimately contributing to a better understanding of calcium and phosphate metabolism. Additionally, the ability to express and purify PTH1R as a recombinant protein opens up possibilities for high-throughput screening of potential therapeutic agents. Therefore, research on PTH1R recombinant proteins not only contributes to the fundamental understanding of receptor biology but also holds promise for clinical applications aimed at treating bone-related diseases.
