Analytical Data
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Gene name
SLC11A2
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简介
The SLC11A2 protein is a proton-coupled metal ion symporter that selectively transports divalent metal cations, including Cd(2+), Fe(2+), Co(2+), Mn(2+), and less Significant Zn(2+), Ni(2+), VO(2+), the stoichiometric ratio of protons and metal ions is 1:1. It is critical for iron homeostasis, regulating intestinal Fe(2+) absorption, transporting TF-associated endosomal Fe(2+), and promoting Fe(2+) and Mn(2+) entry into mitochondria. SLC11A2 Protein, Human (HEK293, His, MBP, FLAG) is the recombinant human-derived SLC11A2 protein, expressed by HEK293 , with N-MBP, C-Flag, N-8*His labeled tag.
- Application
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Alternative Names
SLC11A2; Natural resistance-associated macrophage protein 2; NRAMP 2; Divalent cation transporter 1; Divalent metal transporter 1; DMT-1; Solute carrier family 11 member 2
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Species
Human
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Source
HEK293
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Tag
N-MBP;C-Flag;N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49281
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Expression Region
V2-R568
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SLC11A2, also known as solute carrier family 11 member 2, plays a crucial role in iron homeostasis and has garnered attention due to its involvement in various physiological and pathological conditions. This protein functions primarily as a metal ion transporter, facilitating the uptake of divalent metal ions such as iron and manganese into cells. Dysregulation or mutations in the SLC11A2 gene have been linked to disorders such as iron overload syndromes and certain anemias, highlighting its importance in hematological health. Research into the recombinant expression of SLC11A2 protein has enabled scientists to study its structure, function, and interactions in a controlled environment, advancing our understanding of its role in iron metabolism and the mechanisms underlying related diseases. By using recombinant DNA technology, researchers can produce large quantities of SLC11A2, allowing for detailed biochemical analyses, structural studies via techniques like X-ray crystallography, and the development of inhibitors or modulators that may have therapeutic potential. Overall, SLC11A2 remains a significant target in the field of molecular biology and medicine, as further investigations could lead to novel treatments for iron-related disorders and insights into the complex regulation of mineral transport in human health.
