Analytical Data
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Gene name
SLC29A1
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简介
The SLC29A1 protein is an important uniporter that actively promotes the transport of nucleosides and nucleobases and maintains cellular homeostasis of essential compounds. As a Na(+)-independent transporter, SLC29A1 efficiently transports a series of nucleosides and nucleobases, including adenosine, guanosine, inosine, uridine, thymidine, cytidine, hypoxanthine, adenine, guanine, Thymine and uracil. SLC29A1 Protein, Human (sf9, His, MBP, FLAG) is the recombinant human-derived SLC29A1 protein, expressed by sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag.
- Application
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Species
Human
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Source
Baculovirus
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Tag
N-MBP;C-Flag;N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99808
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Expression Region
T2-V456
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Protein Length
Full Length of Mature Protein
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SLC29A1, also known as the human nucleoside transport protein ENT1, is a member of the solute carrier family that plays a crucial role in the transport of nucleosides across cell membranes. This protein is particularly important in the context of cellular nucleoside uptake and has implications for various physiological processes, including cell proliferation and apoptosis. Research has indicated that SLC29A1 is involved in the pharmacokinetics of nucleoside analogs used in antiviral and anticancer therapies, making it a significant target for studying drug resistance mechanisms. Its dysfunction is associated with various diseases, including some forms of leukemia and genetic disorders. The production of recombinant SLC29A1 protein allows researchers to probe its structure-function relationships and understand its transport mechanisms in detail. By employing techniques such as site-directed mutagenesis, X-ray crystallography, and functional assays in heterologous systems, scientists aim to elucidate the molecular basis of nucleoside transport. Insights gained from these studies can potentially lead to the development of novel therapeutic strategies that enhance drug efficacy by modulating SLC29A1 activity, ultimately contributing to improved treatment regimens for patients affected by conditions linked to this transporter.
