Analytical Data
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基因名
STIM1
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简介
STIM1 (stromal interacting molecule 1) coordinates store-operated Ca(2+) entry (SOCE) and acts as a Ca(2+) sensor in the endoplasmic reticulum. Upon Ca(2+) depletion, STIM1 translocates to the plasma membrane and activates the Ca(2+)-releasing activated Ca(2+) (CRAC) channel subunit ORAI1. STIM1 Protein, Human (HEK293, His) is the recombinant human-derived STIM1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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别名
Stromal interaction molecule 1; STIM1; GOK
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q13586
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表达区间
L23-D213
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氨基酸序列
LSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKD
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蛋白长度
Extracellular Domain
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分子量
27-34 kDa. The protein migrates as 27-34 kDa under reducing SDS-PAGE due to glycosylation.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
STIM1, or stromal interaction molecule 1, is a crucial protein that plays a significant role in regulating calcium homeostasis within cells, particularly in the context of calcium signaling initiated by store-operated calcium entry (SOCE). It is primarily found in the endoplasmic reticulum (ER) and functions as a sensor for calcium levels in this organelle. When calcium stores are depleted, STIM1 undergoes a conformational change and translocates to the ER-plasma membrane junctions, where it interacts with and activates calcium channels such as Orai1. This process is vital for various cellular functions, including muscle contraction, gene expression, and cell proliferation. Dysregulation of STIM1 and SOCE has been implicated in several diseases, including immune disorders, neurodegenerative diseases, and cancer, making it an important target for therapeutic intervention. Research involving recombinant STIM1 protein is essential for understanding its structure-function relationship, identifying potential drug targets, and elucidating the mechanisms underlying calcium signaling pathways. Through techniques such as crystallography and cryo-electron microscopy, scientists can investigate the molecular details of STIM1's interactions and regulatory mechanisms. Overall, studying STIM1 not only provides insights into fundamental cellular processes but also holds promise for developing new strategies in the treatment of STIM1-related pathologies.
