Analytical Data
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Gene name
EXOSC8
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简介
EXOSC8 is a non-catalytic RNA exosome complex component that is critical for 3'->5' exoribonuclease activity and affects a variety of cellular RNA processing and degradation. In the nucleus, it helps stabilize the maturation of RNA species and eliminates processing by-products, non-coding transcripts and defective mRNAs, thereby preventing their export to the cytoplasm. EXOSC8 Protein, Human (His, Strep) is the recombinant human-derived EXOSC8 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Application
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Alternative Names
EXOSC8; Exosome complex component RRP43; Exosome component 8; Opa-interacting protein 2; OIP-2; Ribosomal RNA-processing protein 43; p9
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Species
Human
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Source
E. coli
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Tag
N-StrepⅡ;N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96B26
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Expression Region
M1-K276
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EXOSC8, a pivotal component of the exosome complex, is involved in RNA degradation and processing, playing a crucial role in maintaining cellular RNA homeostasis. Research surrounding EXOSC8 has gained importance due to its implications in various cellular processes, including the regulation of gene expression and responses to stress. Mutations in the EXOSC8 gene have been associated with the rare genetic disorder schwachman-diamond syndrome, which is characterized by bone marrow failure, pancreatic insufficiency, and skeletal abnormalities. Understanding the structure and function of EXOSC8 through recombinant protein studies can illuminate its role in RNA metabolism and the pathophysiology of related diseases. By employing techniques such as protein expression, purification, and functional assays, researchers aim to dissect the biochemical properties of EXOSC8, assess its interaction with other exosome components, and explore how its dysfunction leads to disease phenotypes. Ultimately, investigations into EXOSC8 recombinant protein could not only augment our understanding of basic cellular processes but also pave the way for novel therapeutic strategies targeting the molecular underpinnings of RNA degradation and related disorders.
