Analytical Data
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Gene name
IFI16
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简介
The IFI16 protein prefers supercoiled and cruciform DNA structures, regulates transcription, and may act as a repressor protein, affecting hematopoietic differentiation and controlling cell proliferation. It regulates key cell cycle factors such as p53/TP53 and participates in the energy-dependent ATM/AMPK/TP53 pathway to regulate autophagy. IFI16 Protein, Human (His, Strep) is the recombinant human-derived IFI16 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Application
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Alternative Names
IFI16; Gamma-interferon-inducible protein 16; Ifi-16; Interferon-inducible myeloid differentiation transcriptional activator
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Species
Human
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Source
E. coli
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Tag
N-StrepⅡ;N-6*His;C-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16666
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Expression Region
M1-F729
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
IFI16 (Interferon Gamma-Inducible Protein 16) is a member of the HIN-200 protein family and plays a crucial role in the innate immune response, particularly in recognizing and responding to viral infections. Research on IFI16 has gained prominence due to its ability to detect foreign DNA within the cytoplasm and nuclei of cells, acting as a sensor for viral genomes and triggering inflammatory responses. This protein has been implicated in various cellular processes, including the regulation of gene expression and the modulation of apoptotic pathways. Additionally, IFI16's role in autoimmune diseases, particularly systemic lupus erythematosus (SLE), has prompted further investigation into its function and potential as a therapeutic target. The ability to produce recombinant IFI16 has facilitated detailed studies into its structure-function relationships, interaction with other proteins, and pathways involved in immune signaling. Understanding IFI16's mechanisms can aid in developing new strategies for treating viral infections and autoimmune conditions, highlighting its significance in immunological research.
