Analytical Data
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Gene name
Histone H1
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简介
Histone H1 proteins, particularly the H1.0 variant, are crucial for condensing nucleosome chains, organizing and compacting chromatin. H1.0 is prevalent in cells undergoing terminal differentiation or with low cell division rates. Histone H1 Protein, Human (His) is the recombinant human-derived Histone H1 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Histone H1.0; Histone H1(0); H1-0; H1F0; H1FV
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P07305-1
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Expression Region
M1-K194
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Protein Length
Full Length of Isoform-1
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Molecular Weight
27 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Histone H1 is a pivotal component of the chromatin structure, playing a crucial role in the packaging of DNA into higher-order structures and regulating gene expression. As a member of the linker histone family, H1 binds to the nucleosome, facilitating the formation of chromatin fibers and contributing to the overall stability and compaction of the chromatin. Research on recombinant histone H1 has gained momentum due to its potential applications in understanding chromatin dynamics, epigenetic regulation, and cellular processes such as transcription and replication. The ability to produce recombinant H1 allows for the investigation of its specific functions, interactions with DNA, and influence on chromatin architecture in a controlled setting. Furthermore, studying H1 in recombinant forms aids in elucidating the mechanisms by which post-translational modifications of histones contribute to the regulation of chromatin states and gene expression. Given the critical role of histone H1 in cellular processes and its implications in disease, including cancer and developmental disorders, ongoing research is focused on manipulating H1 expression and studying its effects on cellular function and chromatin remodeling. Understanding the complexities of histone H1 dynamics and interactions at the molecular level could provide insights into targeted therapeutic strategies for various diseases linked to chromatin dysregulation.
