Analytical Data
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Gene name
NAMPT
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简介
NAMPT protein catalyzes the synthesis of nicotinamide mononucleotide, a key intermediate in NAD biosynthesis. NAMPT Protein, Human (sf9, His, GST) is the recombinant human-derived NAMPT protein, expressed by Sf9 insect cells, with N-His and N-GST labeled tag.
- Application
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Alternative Names
rHuNicotinamide phosphoribosyltransferase/NAMPT, His; Pre-B cell-enhancing factor; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Visfatin; NAMPT; PBEF; PBEF1
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P43490
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Expression Region
M1-H491
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AA Sequence
MNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH
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Protein Length
Full Length
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Molecular Weight
75 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Nicotinamide adenine dinucleotide (NAD) metabolism plays a crucial role in various biological processes, and NAMPT (Nicotinamide Phosphoribosyltransferase) is a key enzyme in this pathway, catalyzing the conversion of nicotinamide to nicotinamide mononucleotide, a precursor of NAD. Research on NAMPT has gained significant attention due to its implications in cellular energy metabolism, aging, and various diseases, including cancer, diabetes, and neurodegenerative disorders. Dysregulation of NAMPT expression and activity has been linked to altered NAD levels, which can affect cellular functions such as DNA repair, mitochondrial function, and apoptosis. As a result, NAMPT is considered a potential therapeutic target for modulating NAD levels and improving metabolic health. Studies focusing on the purification and characterization of recombinant NAMPT proteins have facilitated a better understanding of its enzymatic mechanisms, substrate specificity, and interactions with other cellular pathways. This has opened avenues for the development of NAMPT inhibitors or activators that could be used in therapeutic contexts to address diseases associated with altered NAD metabolism.
