Analytical Data
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Gene name
PGK2
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简介
The PGK2 protein is indispensable for sperm motility and male fertility, and studies have confirmed the critical role of the PGK2 protein in these processes. Its presence is essential to ensure the normal movement and function of sperm. PGK2 Protein, Human (sf9, His) is the recombinant human-derived PGK2 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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Alternative Names
Phosphoglycerate kinase 2; PGK2; PGKB
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Species
Human
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Source
Baculovirus
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P07205
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Expression Region
S2-M417
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Protein Length
Full Length of Mature Protein
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Molecular Weight
43.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PGK2, or phosphoglycerate kinase 2, is a glycolytic enzyme primarily expressed in the male germline and has garnered significant attention due to its potential roles in spermatogenesis and male fertility. Unlike PGK1, which is ubiquitous and involved in energy metabolism across various tissues, PGK2 is specifically localized in the testes, making it a subject of interest in reproductive biology. Research indicates that PGK2 plays a critical role in providing the necessary ATP for sperm maturation and motility, thus impacting male reproductive health. Abnormal expression of PGK2 has been linked to infertility in males, highlighting its significance as a biomarker for reproductive disorders. Additionally, PGK2 has been explored for its potential role in cancer, particularly in testicular germ cell tumors, where its expression may correlate with tumor progression. The study of recombinant PGK2 protein has enabled researchers to investigate its biochemical properties and interactions at a molecular level, providing insight into its enzymatic function and regulatory mechanisms. Furthermore, the development of PGK2 inhibitors could open new avenues for therapeutic interventions in both reproductive health and cancer treatment. Overall, the ongoing research into PGK2 not only enhances our understanding of male fertility but also elevates its relevance in biomedical research and potential clinical applications.
