Analytical Data
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基因名
Acetyl-CoA synthetase 1/AceCS
- Application
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别名
Acetate--CoA ligase Acetyl-CoA synthetase Short name:ACS Short name:AceCS
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种属
Mouse
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表达系统
E. coli
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标签
N- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9QXG4
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表达区间
1-701aa
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分子量
82.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Acetyl-CoA synthetase 1 (AceCS1) is a crucial enzyme involved in the metabolism of acetate, converting it into acetyl-CoA, a fundamental building block for various biosynthetic pathways, including lipid synthesis and energy production. In mammals, AceCS1 plays a significant role in cellular energy metabolism and is particularly important in tissues such as the liver, brain, and muscle. The study of recombinant AceCS1 protein is vital for understanding its structure-function relationships, regulatory mechanisms, and physiological roles in metabolic pathways. Additionally, insights into the enzyme's activity and regulation could have implications for metabolic disorders, cancer, and other diseases where acetate metabolism is disrupted. By expressing and purifying recombinant AceCS1, researchers can explore its kinetic properties, understand its interactions with substrates and inhibitors, and investigate its potential as a therapeutic target. Overall, the research on recombinant AceCS1 not only contributes to our fundamental knowledge of metabolic regulation but also opens up avenues for developing novel strategies for disease intervention.
