Analytical Data
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Gene name
RUVBL1
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简介
The RUVBL1 protein has DNA-stimulated ATPase and DNA helicase activities and can hexamerize for ATP hydrolysis. It is a member of the NuA4 complex and contributes to histone acetylation to activate genes. RUVBL1 Protein, Human (sf9, His) is the recombinant human-derived RUVBL1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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Alternative Names
RuvB-like 1; ECP-54; NMP 238; Pontin 52; TIP49a; TAP54-alpha; RUVBL1; INO80H
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Species
Human
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Source
Baculovirus
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y265
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Expression Region
M1-K456
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Protein Length
Full Length
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Molecular Weight
57 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RUVBL1, a member of the RUVBL/RUVBL1/2 family of ATP-dependent helicases, plays a pivotal role in various cellular processes, including transcription regulation, DNA repair, and chromatin remodeling. Its involvement in critical biological functions has positioned RUVBL1 as a significant player in cancer biology and other diseases. Elevated expressions of RUVBL1 have been observed in multiple cancer types, suggesting its function in promoting cell proliferation and survival, making it a potential therapeutic target. Research has indicated that RUVBL1 interacts with several key protein complexes, influencing pathways that regulate cell cycle progression and apoptosis. Additionally, RUVBL1 has been implicated in the maintenance of cellular homeostasis under stress conditions, revealing its potential role in oncogenesis. Recent studies employing recombinant protein techniques have aimed to elucidate RUVBL1's structural properties, functional dynamics, and interaction networks, providing insights into its mechanisms of action. Understanding these aspects is crucial for developing targeted therapies that could disrupt its function in cancer cells and contribute to more effective treatment strategies. Consequently, the recombinant expression and characterization of RUVBL1 not only advance our knowledge of its biological roles but also underscore its potential as a target for innovative cancer therapies.
