Analytical Data
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Gene name
Alanyl-tRNA synthetase
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简介
The Alanyl-tRNA synthetase protein facilitates a two-step process, activating alanine with ATP to form Ala-AMP and transferring it to the acceptor end of tRNA(Ala). Additionally, it corrects incorrectly charged tRNA(Ala) through its editing domain. Alanyl-tRNA synthetase Protein, Human (sf9, His) is the recombinant human-derived Alanyl-tRNA synthetase protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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Alternative Names
Alanine--tRNA ligase, cytoplasmic; AlaRS; AARS1; AARS
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Species
Human
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Source
Baculovirus
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Tag
N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49588
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Expression Region
M1-N968
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Protein Length
Full Length
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Molecular Weight
109.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Alanyl-tRNA synthetase (AlaRS) is a crucial enzyme in the process of protein biosynthesis, responsible for the aminoacylation of tRNA with the amino acid alanine. The accurate functioning of AlaRS is essential for the fidelity of protein translation, as it ensures that the correct amino acid is incorporated into the growing polypeptide chain. Given its vital role in cellular metabolism and protein synthesis, AlaRS has garnered significant attention in research, particularly in the context of antibiotic resistance and the development of novel therapeutic agents. Recombinant expression of AlaRS allows for detailed biochemical studies and structural analyses, facilitating an understanding of its catalytic mechanisms and substrate specificity. By engineering AlaRS from various organisms, scientists aim to elucidate the evolutionary adaptations of this enzyme and its interaction with tRNA and other translation components. Furthermore, the study of AlaRS can provide insights into the development of innovative biotechnological applications, such as in the production of synthetic peptides and proteins with non-canonical amino acids. Overall, the research on recombinant AlaRS not only enhances our comprehension of fundamental biological processes but also holds promising implications for medical and industrial fields.
