Analytical Data
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Gene name
HIST2H2BE
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简介
The HIST2H2BE protein is a core component of nucleosomes, key structures in chromatin organization that wrap and compact DNA, controlling its accessibility to cellular machinery based on DNA templates. This integral role of histones, including HIST2H2BE, extends to transcriptional regulation, DNA repair, DNA replication, and chromosomal stability. HIST2H2BE Protein, Human is the recombinant human-derived HIST2H2BE protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
Histone H2B type 2-E; Histone H2B-GL105; H2B/q; H2BC21; H2BFQ
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16778
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Expression Region
M1-K126
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Protein Length
Full Length
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Molecular Weight
16 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HIST2H2BE, a member of the histone H2B family, plays a crucial role in the packaging of DNA into chromatin, thus influencing gene expression and cellular processes. As a highly conserved protein, it is essential for maintaining chromatin structure and stability, which in turn affects various biological functions, including DNA replication, repair, and transcription. Recent research has highlighted the significance of histone variants like HIST2H2BE in epigenetic regulation, with implications for development, cellular differentiation, and disease states such as cancer. The study of HIST2H2BE and its reconstituted versions provides valuable insights into the dynamics of chromatin and allows for the exploration of post-translational modifications that regulate histone function. Understanding the functional properties of HIST2H2BE is critical for elucidating its role in the epigenetic landscape and for developing potential therapeutic strategies targeting chromatin-related disorders. The production of recombinant HIST2H2BE proteins facilitates a deeper investigation into their biochemical properties, interactions with other histones, and effects on chromatin organization and gene regulation, which can advance our understanding of fundamental cellular mechanisms.
