Recombinant Dog Beta-galactosidase/GLB1 Protein，His

Beta-galactosidase, also known as GLB1, is an essential enzyme involved in the hydrolysis of beta-galactosides into monosaccharides, playing a critical role in various biological processes, including lactose metabolism. Its significance is underscored by its association with several diseases, most notably Morquio B syndrome, a lysosomal storage disorder caused by GLB1 mutations leading to the accumulation of glycosaminoglycans. The production of recombinant Beta-galactosidase has gained increasing attention in both research and therapeutic contexts, as it provides a means to study the enzyme's structure-function relationships and its role in cellular pathways. Moreover, recombinant GLB1 has potential therapeutic applications, including enzyme replacement therapy for affected individuals. Advances in recombinant DNA technology have facilitated the efficient expression and purification of GLB1 in various host systems, enhancing its availability for in-depth biochemical characterization. This research aims to elucidate the functional implications of different mutations and to develop strategies for the treatment of GLB1-related disorders. In addition, understanding the enzyme's kinetics and substrate specificity can pave the way for novel biotechnological applications in food and pharmaceutical industries, emphasizing the importance of continued research in this field.