Analytical Data
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Gene name
CENPE
- Application
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Alternative Names
Centromere-associated protein E; Centromere protein E; CENP-E; Kinesin-7; Kinesin-related protein CENPE; CENPE; Homo sapiens; Human; CENP-E; Developmental protein; Motor protein
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q02224
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Expression Region
M1-S339
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Protein Length
Partial
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Molecular Weight
38.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CENPE (Centromere Protein E) is a crucial component in the kinetochore complex, playing a vital role in the proper segregation of chromosomes during cell division. As a motor protein, CENPE is responsible for facilitating the movement of chromosomes along the spindle apparatus, ensuring their accurate alignment and separation. Dysregulation or mutations in the CENPE gene have been implicated in various cancers, making it a significant target for therapeutic intervention. Research into CENPE has intensified due to its potential as a biomarker for cancer progression and as a target for novel drug development. The recombinant expression of CENPE protein allows for in-depth studies of its structure, function, and interaction with other cellular components. By producing CENPE in a controlled laboratory setting, researchers can investigate its mechanistic role in chromosome dynamics, explore its involvement in the cell cycle, and assess how alterations in its activity can lead to oncogenesis. Furthermore, recombinant CENPE provides an invaluable tool for high-throughput screening of small molecules that may modulate its activity, paving the way for potential cancer therapies. Understanding CENPE's dynamics and regulatory mechanisms could ultimately enhance our understanding of chromosomal instability in cancer and contribute to the development of targeted interventions aimed at improving patient outcomes.
