Analytical Data
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基因名
COX1
- Application
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别名
MT-CO1; MTCO1; COI; COXI; Mitochondrially Encoded Cytochrome C Oxidase I; Cytochrome c oxidase polypeptide I
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种属
Rat
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表达系统
E. coli
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标签
Two N- s, His- & SUMO-
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P05503
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表达区间
Lys479~Lys514
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分子量
20kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
COX1, or cytochrome c oxidase subunit 1, is a critical enzyme in the mitochondrial respiratory chain, playing a pivotal role in cellular energy production through ATP synthesis. It is a key component of complex IV, facilitating the transfer of electrons from cytochrome c to molecular oxygen, which ultimately helps in the formation of water and the production of an electrochemical gradient essential for ATP generation. Research on COX1 recombinant proteins has gained significant attention due to their implications in understanding mitochondrial diseases, aging, and metabolic disorders. Recombinant COX1 allows scientists to study the structure-function relationships of this enzyme, assess the impacts of genetic mutations, and explore the enzyme's interactions with various substrates and inhibitors. Moreover, it serves as a valuable tool in the development of targeted therapies for conditions associated with mitochondrial dysfunction. Additionally, investigations into COX1 may contribute to advancements in synthetic biology and bioengineering, where engineered mitochondrial enzymes can enhance bioenergetic processes in both microbial and mammalian systems. Understanding the dynamics of COX1 through recombinant protein studies enhances our grasp of its biophysical properties, regulatory mechanisms, and roles in various pathological states, thus paving the way for novel therapeutic strategies aimed at combating diseases tied to mitochondrial dysfunction.












