Analytical Data
-
Gene name
MBD1
- Application
-
Alternative Names
CXXC3; PCM1; RFT; CXXC-type zinc finger protein 3; Methyl-CpG-binding protein MBD1; Protein containing methyl-CpG-binding domain 1
-
Species
Human
-
Source
E. coli
-
Tag
N-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9UIS9
-
Expression Region
Met1~Leu216
-
Molecular Weight
28kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MBD1 (Methyl-CpG Binding Domain Protein 1) is a crucial player in the epigenetic regulation of gene expression, primarily through its role in recognizing and binding to methylated DNA. This protein is part of the MBD family, which contributes to transcriptional repression by recruiting co-repressors and chromatin-modifying enzymes to methylated regions of the genome. Research on MBD1 has gained momentum due to its implications in various biological processes, including development, differentiation, and cellular response to external stimuli. Moreover, dysregulation of MBD1 has been linked to several diseases, including cancer and neurological disorders, making it a significant focus for therapeutic interventions. Scientists are particularly interested in the structural and functional aspects of MBD1, exploring how its conformational changes influence DNA binding and interaction with other proteins. Understanding these mechanisms could provide insights into the broader roles of DNA methylation in gene silencing and epigenetic inheritance. As such, recombinant MBD1 proteins are being studied not only for their potential applications in gene therapy and cancer treatment but also as valuable tools for elucidating the complex regulatory networks that govern gene expression. Overall, the study of MBD1 and its recombinant forms represents a cutting-edge area in molecular biology with vast implications for medicine and therapeutic development.
