Analytical Data
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Gene name
CHD8
- Application
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Alternative Names
HELSNF1; DUPLIN; Helicase With SNF2 Domain 1; ATP-dependent helicase CHD8
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HCK8
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Expression Region
Leu823~Arg1288
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Molecular Weight
58kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHD8 (Chromodomain helicase DNA-binding protein 8) is a highly conserved protein implicated in various biological processes, including chromatin remodeling and gene regulation. Its mutations have been linked to neurodevelopmental disorders, particularly autism spectrum disorder (ASD), making it a significant focus for research in the fields of genetics and developmental biology. CHD8 functions as a chromatin remodeler, influencing the organization of chromatin structure and thereby regulating the expression of genes involved in neuronal development and function. Studies have shown that CHD8 interacts with other key proteins in the transcriptional machinery, highlighting its role in modulating gene networks critical for brain development. The generation of CHD8 recombinant proteins in experimental settings has enabled researchers to investigate its molecular mechanisms, interaction partners, and effects on gene expression more precisely. Understanding CHD8's function and the consequences of its dysregulation is crucial for deciphering the biological pathways affected in ASD, suggesting potential therapeutic targets for intervention. The ongoing research aims to elucidate the structure-function relationship of CHD8 and its role in neurodevelopmental processes, providing insights into the etiology of related disorders and advancing our knowledge of chromatin dynamics in health and disease.
