Analytical Data
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Gene name
S100A13
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简介
S100A13 Protein, acting as a homodimer, facilitates the export of signal peptide-lacking proteins through an alternative pathway, binding two calcium ions and one copper ion per subunit. It is crucial for the copper-dependent stress-induced export of IL1A and FGF1, with the calcium-free form binding to phosphatidylserine-containing lipid vesicles. S100A13 is part of a copper-dependent multiprotein complex, interacting with FGF1, SYT1, and IL1A. S100A13 Protein, Mouse (N-His) is the recombinant mouse-derived S100A13 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Biological Activity
Measured by its ability to enhance the outgrowth of SH-SY5Y cells. The ED50 of this effect is 1.075 μg/mL, corresponding to a specific activity is 930.233 units/mg. Measured by its ability to enhance the outgrowth of SH-SY5Y cells. The ED50 of this effect is 1.075 μg/mL, corresponding to a specific activity is 930.233 units/mg.
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Alternative Names
Protein S100-A13; S100A13; S100 calcium-binding protein A13
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Species
Mouse
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P97352
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Expression Region
M1-K98
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Protein Length
Full Length
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Molecular Weight
12 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
S100A13 is a member of the S100 protein family, which is characterized by its ability to bind calcium and zinc ions, thereby playing crucial roles in various cellular processes, including cell proliferation, differentiation, and apoptosis. This protein is primarily expressed in the cytoplasm and is known to be involved in the regulation of inflammatory responses, stress signaling, and intracellular transport. Research has indicated that S100A13 can interact with other proteins, influencing signaling pathways such as those related to cancer progression and metastasis. As a result, understanding the structure and function of S100A13 is essential for elucidating its roles in pathological conditions, including cancer and neurodegenerative diseases. The recombinant expression of S100A13 allows for detailed biochemical and biophysical studies, enabling scientists to explore its interactions, post-translational modifications, and potential as a therapeutic target. Given its involvement in critical cellular functions, there is a growing interest in S100A13 as a biomarker for disease diagnosis and prognosis, as well as a potential target for novel therapeutic strategies. The ongoing research aims to uncover the precise mechanisms through which S100A13 contributes to disease, paving the way for the development of interventions that could mitigate its detrimental effects.
