Analytical Data
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基因名
GM-CSF
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简介
The CSF2 protein is a cytokine that plays a critical role in the regulation of immune responses and hematopoiesis. It stimulates the production and differentiation of white blood cells, including macrophages and granulocytes. GM-CSF Protein, Canine is the recombinant canine-derived GM-CSF protein, expressed by E. coli , with tag free.
- Application
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生物活性
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 7.016 ng/ml, corresponding to a specific activity is 1.42×10^5 units/mg. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 7.016 ng/mL, corresponding to a specific activity is 1.42×105 units/mg.
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别名
colony stimulating factor 2; CSF2; Granulocyte Macrophage Growth Factor
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种属
Canine
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P48749
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表达区间
A18-K144
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蛋白长度
Full Length of Mature Protein
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分子量
15 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pivotal cytokine that plays a crucial role in the regulation of immune responses, particularly in the proliferation, differentiation, and activation of myeloid lineage cells, such as neutrophils and monocytes. Research on GM-CSF has gained significant attention due to its implications in various pathophysiological conditions, including autoimmune diseases, cancer, and infections. Recombinant GM-CSF (rGM-CSF) has been developed to utilize its therapeutic potential, showing promise in enhancing immune reconstitution in patients undergoing chemotherapy or bone marrow transplantation. It has also been investigated for its role in vaccine adjuvants and immunotherapy, as it can boost the immune system's ability to respond to pathogens and tumors. Furthermore, the unique molecular properties of rGM-CSF, including its ability to modulate immune cell activity, have sparked interest in studying its mechanisms of action and optimizing its delivery in clinical applications. However, despite the encouraging findings, challenges remain regarding its dosage, safety profile, and long-term effects, warranting continued exploration of rGM-CSF to maximize its therapeutic efficacy and minimize adverse reactions. Overall, GM-CSF recombinant protein research represents a dynamic field with the potential to significantly advance our understanding and treatment of diverse immune-related disorders.
