Analytical Data
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Gene name
Cereblon-DDB1-Cul4A-Rbx1 Complex
- Application
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Alternative Names
ROC1; RNF75; Regulator Of Cullins 1; E3 ubiquitin-protein ligase RBX1; Protein ZYP; RING finger protein 75; Regulator of cullins 1
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P62877
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Expression Region
Met1~His108
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Molecular Weight
16kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The Cereblon-DDB1-Cul4A-Rbx1 complex is a crucial E3 ubiquitin ligase involved in protein degradation and cellular regulation. This multi-protein complex plays a significant role in the ubiquitin-proteasome system, where it mediates the tagging of substrates with ubiquitin for subsequent degradation. Cereblon, the substrate receptor of the complex, has gained attention due to its involvement in the action of thalidomide and its analogs, which are used in the treatment of multiple myeloma. Research has shown that Cereblon exerts its effects by regulating the stability and degradation of key proteins, influencing various cellular processes such as cell cycle progression, differentiation, and immune response. DDB1 serves as a scaffold that links Cereblon to the Cul4A-Rbx1 core, facilitating substrate recognition and ubiquitination. Understanding the structural and functional dynamics of this complex is vital for elucidating its role in diseases, particularly in cancer. Recent studies employing recombinant protein techniques have enabled the detailed characterization of the Cereblon-DDB1-Cul4A-Rbx1 complex, paving the way for potential therapeutic interventions targeting this pathway. This research is essential for developing new strategies to modulate protein degradation in diseases, offering insights into the complex regulatory mechanisms governing cellular homeostasis.
