Analytical Data
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Gene name
GAPDHS
- Application
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Alternative Names
Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2 Short name:GAPDH-2
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Species
Human
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Source
Baculovirus
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14556
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Expression Region
69-408aa
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Molecular Weight
39.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GAPDH, or glyceraldehyde-3-phosphate dehydrogenase, is a key enzyme in the glycolytic pathway, playing a crucial role in energy metabolism by catalyzing the conversion of glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate. Beyond its metabolic functions, GAPDH has been implicated in various cellular processes, including apoptosis, transcription regulation, and DNA repair, highlighting its importance in both normal physiology and pathophysiological conditions. The research on recombinant GAPDH proteins has gained traction due to the enzyme’s potential as a biomarker for several diseases, including neurodegenerative disorders and cancer. Furthermore, recombinant GAPDH has been utilized in various biotechnological applications, such as in biosensors and therapeutic agents, owing to its biochemical properties and stability. Scientists are focusing on the production and characterization of recombinant GAPDH to better understand its functional roles and regulatory mechanisms. By employing techniques such as molecular cloning and protein expression in host systems like E. coli, researchers aim to produce large quantities of pure GAPDH for in-depth studies. This research not only enhances our understanding of GAPDH's multifaceted roles in cellular metabolism and disease but also paves the way for potential applications in diagnostics and therapeutics, making it a significant area of investigation in molecular biology and biotechnology.
