Analytical Data
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基因名
FSHR
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简介
FSHR, a G protein-coupled receptor, specifically recognizes follitropin (FSH) and activates PI3K-AKT and ERK1/ERK2 pathways by promoting cAMP production. Operating as a homotrimer, FSHR binds the heterodimeric FSH hormone, forming a functional unit for signal transduction. Its regulatory mechanisms involve interaction with ARRB2, and independently of FSH stimulation, it engages with APPL2, demonstrating versatility in diverse cellular contexts. FSHR Protein, Human (His) is the recombinant human-derived FSHR protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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生物活性
Immobilized Human FSH at 2 μg/mL (100 μL/well) can bind Human FSHR. The ED50 for this effect is 0.265 μg/mL. Immobilized Human FSH at 2 μg/mL (100 μL/well) can bind Human FSHR. The ED50 for this effect is 0.265 μg/mL.
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P23945-1
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表达区间
C18-R366
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氨基酸序列
CHHRICHCSNRVFLCQESKVTEIPSDLPRNAIELRFVLTKLRVIQKGAFSGFGDLEKIEISQNDVLEVIEADVFSNLPKLHEIRIEKANNLLYINPEAFQNLPNLQYLLISNTGIKHLPDVHKIHSLQKVLLDIQDNINIHTIERNSFVGLSFESVILWLNKNGIQEIHNCAFNGTQLDELNLSDNNNLEELPNDVFHGASGPVILDISRTRIHSLPSYGLENLKKLRARSTYNLKKLPTLEKLVALMEASLTYPSHCCAFANWRRQISELHPICNKSILRQEVDYMTQARGQRSSLAEDNESSYSRGFDMTYTEFDYDLCNEVVDVTCSPKPDAFNPCEDIMGYNILR
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蛋白长度
Extracellular Domain
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分子量
47-51 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Follicle-stimulating hormone receptor (FSHR) is a critical protein involved in the regulation of reproductive processes by mediating the actions of follicle-stimulating hormone (FSH). FSH plays a vital role in the development of ovarian follicles in females and spermatogenesis in males. Abnormalities in FSHR signaling can lead to various reproductive disorders, such as infertility, ovarian hyperstimulation syndrome, and hypogonadism. Given its central role in reproductive health, FSHR has become a significant target for therapeutic interventions. Researchers have focused on the generation of recombinant FSHR proteins to facilitate structural and functional studies, enabling a better understanding of its signaling mechanisms. These reconstituted proteins can be used to study receptor pharmacology, develop novel contraceptive methods, and create treatments for fertility-related issues. Recent advancements in protein engineering techniques have improved the yield and functionality of these recombinant proteins, allowing for high-precision studies that could lead to breakthroughs in reproductive health therapies. The ongoing exploration of FSHR and its interactions continues to provide valuable insights into reproductive biology and potential clinical applications.
