Analytical Data
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Gene name
Frizzled-7
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简介
Frizzled-7 (FZD7) is a Wnt receptor that primarily activates the β-catenin classical pathway, involving Disheveled protein, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene activation. Some family members exhibit a second PKC and calcium flux signaling pathway, but the integration is unclear. Frizzled-7/FZD7 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-7/FZD7 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
FZD7; Frizzled-7; FzE3; Fz-7; hFz7
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75084
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Expression Region
Q33-L185
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Protein Length
Partial
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Molecular Weight
23-33 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-7 (Fz7) is a member of the Frizzled family of proteins, which are integral to the Wnt signaling pathway, a crucial mechanism in various developmental processes and tissue homeostasis. In recent years, research has focused on Fz7 due to its significant role in the regulation of cellular functions such as cell proliferation, differentiation, and polarity. Dysregulation of Wnt signaling, often mediated by Frizzled receptors like Fz7, has been implicated in numerous diseases, including cancers and developmental disorders. Therefore, understanding the structure and function of Fz7 is paramount for elucidating the molecular mechanisms underpinning these pathologies. The study of Fz7 recombinant proteins has gained traction as it allows researchers to investigate the receptor's binding affinities, signaling capabilities, and interactions with Wnt ligands and other intracellular partners in a controlled environment. Additionally, recombinant Fz7 proteins can serve as valuable tools for the development of therapeutic strategies aimed at modulating Wnt signaling. By employing techniques such as protein expression in heterologous systems and subsequent purification, researchers aim to characterize the biochemical properties of Fz7 further. This knowledge can pave the way for potential interventions in conditions associated with aberrant Wnt signaling, highlighting the importance of Frizzled-7 in both basic biology and clinical applications.
