Analytical Data
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Gene name
RAD1
- Application
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Alternative Names
DNA repair exonuclease rad1 homolog Rad1-like DNA damage checkpoint protein
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Species
Human
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Source
E. coli
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Tag
N- GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60671
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Expression Region
1-282aa
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Molecular Weight
58.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RAD1 (Recombination Activation 1) is a key protein involved in DNA damage response and repair mechanisms, playing a critical role in maintaining genomic stability. It is part of the RAD1-RAD9-HUS1 complex, known as the 911 complex, which is essential for the cellular response to double-strand breaks and other forms of DNA damage. The RAD1 protein aids in the activation of cell cycle checkpoints, ensuring proper DNA repair processes before cell division. Research has shown that alterations in RAD1 function can lead to increased sensitivity to genotoxic agents and a higher likelihood of developing cancer, as it compromises the integrity of the DNA repair machinery. Additionally, RAD1 has been implicated in various cellular processes beyond DNA repair, including apoptosis and the regulation of gene expression. Understanding the structure, function, and regulatory mechanisms of RAD1 and its interactions within the broader context of the DNA damage response network is crucial for developing potential therapeutic strategies aimed at enhancing DNA repair in cancer cells or sensitize them to radiotherapy and chemotherapy. Advances in biochemistry and molecular biology techniques have allowed for the detailed study of RAD1's role in these processes, contributing to a more comprehensive understanding of its implications in human health and disease. Ongoing research aims to elucidate the precise molecular pathways involving RAD1, offering insights into potential interventions for diseases linked to DNA repair deficiencies.
