Analytical Data
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Gene name
RNF212
- Application
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Alternative Names
RING finger protein 212
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Species
Human
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Source
E. coli
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Tag
N- GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q495C1
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Expression Region
1-297aa
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Molecular Weight
60.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RNF212, a RING-type E3 ubiquitin ligase, plays a critical role in the regulation of various cellular processes, including DNA repair, cell cycle progression, and genome stability. Its involvement in meiotic recombination is particularly notable, where it has been shown to facilitate the synapsis of homologous chromosomes and promote the formation of crossovers, essential for proper gametogenesis. Aberrant expression or mutation of RNF212 has been associated with infertility and other reproductive disorders, highlighting its importance in reproductive biology. Recent studies have aimed to elucidate the molecular mechanisms underlying RNF212's function, exploring its interactions with key proteins involved in meiosis and DNA repair pathways. The recombinant expression of RNF212 protein in various model systems has enabled researchers to investigate its enzymatic activity, structural properties, and the effects of specific mutations. Understanding the precise role of RNF212 in cellular processes not only sheds light on fundamental biological mechanisms but also paves the way for potential therapeutic developments in treating fertility issues and other related disorders. This growing body of research underscores the significance of RNF212 as a pivotal player in maintaining genomic integrity and its potential as a biomarker or target for interventions in reproductive health.
