Analytical Data
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基因名
ERK1
- Application
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别名
ERK1；ERK1；PRKM3；Mitogen-activated Protein kinase 3
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种属
Human
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表达系统
E. coli
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标签
N-6His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P27361
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表达区间
2-379aa
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氨基酸序列
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAAAQGGGGGEPRRTEGV GPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAI KKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYI VQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS NLLSNTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSK GYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLN CIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRIT VEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETAR FQPGVLEAP
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分子量
46 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
The research on recombinant ERK1 protein (Extracellular Signal-Regulated Kinase 1) stems from its critical role in cellular signaling pathways, particularly the MAPK/ERK pathway, which is essential for various cellular processes including proliferation, differentiation, and survival. Abnormal regulation of ERK1 is implicated in numerous diseases, including cancer, where its persistent activation drives uncontrolled cell growth and tumor progression. Given its importance, there has been considerable interest in understanding the structure-function relationship of ERK1 and its interactions with other proteins within the signaling cascade. Recombinant technology has enabled the production of ERK1 in a controlled laboratory setting, facilitating detailed biochemical studies, functional assays, and potential therapeutic applications. These studies not only enhance our comprehension of ERK1's role in normal physiology but also pave the way for developing targeted therapies to modulate its activity in pathological conditions. The availability of purified recombinant ERK1 allows researchers to explore its substrate specificity, phosphorylation dynamics, and its influence on downstream effectors, thereby providing crucial insights into the molecular mechanisms underpinning various diseases and identifying novel intervention points for drug development.
