Analytical Data
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Gene name
SPTLC1
- Application
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Alternative Names
SPTLC1；LCB1；Serine palmitoyltransferase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15269
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Expression Region
1-473aa
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AA Sequence
MATATEQWVLVEMVQALYEAPAYHLILEGILILWIIRLLFSKTYKLQERSDLTVKEKEELIEEWQPEPLVPPVPKDHPALNYNIVSGPPSHKTVVNGKECINFASFNFLGLLDNPRVKAAALASLKKYGVGTCGPRGFYGTFDVHLDLEDRLAKFMKTEEAIIYSYGFATIASAIPAYSKRGDIVFVDRAACFAIQKGLQASRSDIKLFKHNDMADLERLLKEQEIEDQKNPRKARVTRRFIVVEGLYMNTGTICPLPELVKLKYKYKARIFLEESLSFGVLGEHGRGVTEHYGINIDDIDLISANMENALASIGGFCCGRSFVIDHQRLSGQGYCFSASLPPLLAAAAIEALNIMEENPGIFAVLKEKCGQIHKALQGISGLKVVGESLSPAFHLQLEESTGSREQDVRLLQEIVDQCMNRSIALTQARYLEKEEKCLPPPSIRVVVTVEQTEEELERAASTIKEVAQAVLL
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Molecular Weight
52.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPTLC1 (serine palmitoyltransferase long chain base subunit 1) is a crucial enzyme in the sphingolipid biosynthetic pathway, which catalyzes the initial step of ceramide production by transferring a fatty acyl-CoA moiety to serine. This enzyme plays a key role in various cellular processes, including membrane integrity, cell signaling, and apoptosis. Mutations in the SPTLC1 gene have been associated with disorders like hereditary sensory and autonomic neuropathy type I (HSAN I) and other sphingolipid-related diseases. Understanding the structure and function of SPTLC1 is essential not only for elucidating its role in these pathological conditions but also for developing potential therapeutic strategies. Recent advances in recombinant protein technology have enabled the production of functional SPTLC1 proteins, facilitating in-depth biochemical studies and structural analyses. These studies aim to unveil the enzyme's mechanistic properties, substrate specificity, and regulation, paving the way for targeted drug discovery and the development of novel treatments for SPTLC1-related disorders.
